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Doses and application methods of Azospirillum brasilense in irrigated upland rice AGRIAMBI
Garcia,Nayara F. S.; Arf,Orivaldo; Portugal,José R.; Peres,Amanda R.; Rodrigues,Mayara; Penteado,Mariele de S..
ABSTRACT The study was carried out in Selvíria-MS, in the 2011/12 and 2012/13 agricultural years, aiming to evaluate the efficiency of Azospirillum brasilense in nitrogen fixation in upland rice, as a function of doses and application methods of the inoculant containing this diazotrophic bacterium. The experimental design was randomized blocks, arranged in a 4 x 4 factorial scheme, with 4 doses of inoculant (control without inoculation, 100, 200 and 300 mL of the commercial product ha-1) and 4 application methods (seed inoculation, application in the sowing furrow, soil spraying after sowing, and foliar spraying at the beginning of plant tillering), with 4 replicates. During the experiment, the agronomic characteristics, production components and yield of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oryza sativa L.; Diazotrophic; Cv. IAC 202; Nitrogen fixation; Grain yield.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662016001100990
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Draft genome sequence of Paraburkholderia tropica Ppe8 strain, a sugarcane endophytic diazotrophic bacterium BJM
Silva,Paula Renata Alves da; Simões-Araújo,Jean Luiz; Vidal,Márcia Soares; Cruz,Leonardo Magalhães; Souza,Emanuel Maltempi de; Baldani,José Ivo.
Abstract Paraburkholderia tropica (syn Burkholderia tropica) are nitrogen-fixing bacteria commonly found in sugarcane. The Paraburkholderia tropica strain Ppe8 is part of the sugarcane inoculant consortium that has a beneficial effect on yield. Here, we report a draft genome sequence of this strain elucidating the mechanisms involved in its interaction mainly with Poaceae. A genome size of approximately 8.75 Mb containing 7844 protein coding genes distributed in 526 subsystems was de novo assembled with ABySS and annotated by RAST. Genes related to the nitrogen fixation process, the secretion systems (I, II, III, IV, and VI), and related to a variety of metabolic traits, such as metabolism of carbohydrates, amino acids, vitamins, and proteins, were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diazotrophic; Next generation sequencing; Nitrogen-fixing; Secretion system.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200210
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Use of endophytic diazotrophic bacteria as a vector to express the cry3A gene from Bacillus thuringiensis BJM
Salles,Joana Falcão; Gitahy,Patrícia de Medeiros; Skøt,Leif; Baldani,José Ivo.
The goal of this study was to evaluate the potential of endophytic diazotrophic bacteria as a vector to express a cry gene from Bacillus thuringiensis, envisaging the control of pests that attack sugarcane plants. The endophytic nitrogen-fixing bacteria Gluconacetobacter diazotrophicus strain BR11281 and Herbaspirillum seropedicae strain BR11335 were used as models. The cry3A gene was transferred by conjugation using a suicide plasmid and the recombinant strains were selected by their ability to fix nitrogen in semi-solid N-free medium. The presence of the cry gene was detected by Southern-blot using an internal fragment of 1.0 kb as a probe. The production of delta-endotoxin by the recombinant H. seropedicae strain was detected by dot blot while for G....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gluconacetobacter diazotrophicus; Herbaspirillum seropedicae; Endophytic bacteria; Diazotrophic; Cry gene.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300001
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